All About Food Sciences & Tech.

OBJECT: To determine the Ph of different solutions using the Ph meter.  REQUIREMENTS: pH Meter Caliberator Apple Juice Coke Glass Electrode THEORY: pH Definition: Measure of acidity or alkalinity on a scale from 0-14, with 7 being neutral. Importance of pH in Food: The pH level of food and its impact on acid-base balance plays an important role in several areas of nutrition, including food safety and medical nutrition therapy.  pH Meter: A pH meter is a scientific instrument that measures the hydrogen-ion activity in water-based solutions , indicating its acidity or alkalinity expressed as pH . Principle of pH Meter: Potentiometric pH meters measure the voltage between two electrodes and display the result converted into the corresponding pH value . Applications of pH Meter: pH meters are used for soil measurements in agriculture, water quality for municipal water supplies, swimming pools, environmental remediation; brewing of wine or beer; manufacturing, healthcare and ...

  OBJECTIVE: Estimation of protein by the Biuret method (Qualitative Analysis)  REQUIREMENTS:  Test tubes  Measuring cylinder  Dropper Reagents: Protein Sample  CuSO4 NaOH  THEORY:  This is the most commonly used method based on the fact that the –CO – NH- (peptide) group of proteins forms a purple complex with copper ions in an alkaline medium. Since all proteins contain the peptide bond. The method is fairly specific and there is little interference from other compounds. The normal color of biuret reagent is blue. The reagent turns violet in the presence of peptide bonds. The chemical bonds that hold amino acids together. The proteins detected must have at least 3 amino acids, which mean that the protein must have at least two peptide bonds.  The biuret test uses an alkaline mixture or reagent, composed of sodium hydroxide and copper sulfate.  The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and sodium hydrox...

Introduction to food technology: Food technology is the application of food science to the selection, preservation, processing, packaging, distribution, and use of safe food. Food scientists study the physical microbiological, and chemical makeup of food.  Lab safety rules: Lab coats Closed Shoes Hair tied No bags, bottles, food item on the table No cell phones Do not stand when not working General Rules: No Food or Drink. Wear Your PPE and Proper Lab Attire. Good Hygiene. Use Proper Storage Containers. Label Your Work Space. Don't Work Alone. Stay Focused and Aware of Your Surroundings. PPE’s: Gloves First Aid box Mask Handling Chemicals: Store chemicals and medicines in a safe way. Store chemicals where children cannot reach them or open them. Add clear labels to packages of chemicals. Avoid storing chemicals in drinking bottles. Dispose of chemicals and medicines in a safe way. Avoid disposing chemicals and medicine on the ground, in rivers, or into sinks, drains or toilets. Avo...